Abstract
Introduction. The formation of adhesions remains one of the dangerous complications that occur in surgery, including in cardiac surgery. This problem is very urgent and far from being resolved. Films and plates (in comparison with gels and solutions) based on biodegradable polymers may be the most promising in solving this problem. Animal gelatin is widely used in medicine. From the point of view of biological safety gelatin from fish raw materials may also be promising. Fish gelatin is a relatively new version of gelatin, which has shown the promise of its use in agriculture and the food industry. The purpose of this work is a comparative assessment of film compositions from animal and fish gelatin in the prevention of postoperative adhesion formation.
Material and methods. Fish gelatin from fish scales of the Volga-Caspian basin (Astrakhan State Technical University, Astrakhan), and highly purified animal gelatin (LLC Mozhelit, Mogilev) were used as a raw material. The medicinal product is an anti-inflammatory herbal preparation olchicine/Kolkhikum-Dispert. The plasticizer is glycerin. The films were sterilized with ethylene oxide. Physical and mechanical characteristics, degradation time, and interaction of samples with native blood in vitro were evaluated. Experimental studies were conducted in vivo on pigs.
Results. Based on the literature data and our own assessment, it was found that the positive quality indicators of fish gelatin correspond to animal gelatin used in the medical industry: good water solubility, film formation, degradation to non-toxic substances, the possibility of sterilization with ethylene oxide, but unlike animal gelatin, fish gelatin is more biocompatible, has increased strength, greater elasticity, increased adhesion to biological tissues and better handling properties.
Conclusions. In vivo experiments on pigs, the effectiveness of film compositions based on fish gelatin in the prevention of adhesion in the pericardial cavity was confirmed.
References
- Ananiev V.N. Nanotechnological matrix of the mechanism of action and drug delivery in the form of gelatin films. Farmastevticheskie Nauki (Pharmaceutical Sciences). 2011; 5: 53–7 (in Russ).
- Bockeria L.A., Novikova S.P., Bockeria O.L., Kostrov V.I., Salokhedinova R.R., Nikolashina L.N. et al. The film compositions based on gelatin structured by different ways. The Bulletin of Bakoulev Center. Cardiovascular Diseases. 2014; 15 (4): 60–72 (in Russ).
- Bockeria L.А., Novikova S.P., Loseva S.V., Shustrova O.V. The method of processing synthetic textile implantable medical devices which is in a contact with blood. Patent RU № 2462273, 2012 (in Russ).
- Abdulgasanov R.A., Bockeria L.A., Novikova S.P., Abdulgasanova M.R., Gazimagomedov Z. I., Galustov D.V., Ordokov A.A. Domestic explants "BASEKS" in reconstructive surgery of the aorta and main arteries. Kompleksnye Problemy Serdechno-Sosudistykh Zabolevaniy (Complex Problems of Cardiovascular Diseases). 2016; 5 (3): 69–74 (in Russ). DOI: 10.17802/2306-1278-2016- 5-3-69-74
- Anuchina N.M., Bockeria L.А., Bockeria О.L., Novikova S.P., Salokhedinova R.R., Shustrova O.V. et al. Analysis of antimicrobial properties and sterility of products for cardiovascular surgery. The Bulletin of Bakoulev Center. Cardiovascular Diseases. 2016; 17 (6, appl.): 240 (in Russ).
- Bockeria L.A., Bockeria O.L., Novikova S.P., Salokhedinova R.R, Shustrova O.V., Samsonova N.N. et al. Polymer materials in cardiology. Polymer compositions of the Center named after A.N. Bakulev for the prevention of postoperative complications in cardiovascular surgery. Khimicheskiy Zhurnal (The Chemical Journal). 2017; 6: 34–5 (in Russ).
- Bockeria L.A., Gorodkov A.Yu., Novikova S.P., Britikov D.V., Fadeev A.A. Advances of experimental cardiosurgery in «A.N. Bakulev Scientific Center for Cardiovascular Surgery» Ministry of Health of Russian Federation for the last 10 years. The Bulletin of Bakoulev Center. Cardiovascular Diseases. 2016; 17 (3): 145–58 (in Russ).
- Novikova S.P., Salokhedinova R.R, Loseva S.V., Nikolashina L.N., Levkina A.Yu. Analysis of the physical-mechanical and structural characteristics of prostheses of blood vessels. Russian Journal of Thoracic and Cardiovascular Surgery. 2012; 4: 27–33 (in Russ).
- Bockeria L.A., Bockeria O.L., Biniashvili M.B., Donakanyan S.A., Novikova S.P., Salokhedinova R.R. et al. In vivo evaluation of the bioresorbable polymeric compositions "ANTISPAY-LAB", "ANTISPAY-LAB M" for the prevention of adhesions complications in the experiment. The Annals of Surgery. 2018; 5: 299–313 (in Russ). DOI: 10.18821/1560-9502-2018-23-5-299-313
- Bockeria L.A., Bockeria O.L., Novikova S.P., Salokhedinova R.R., Nikolashina L.N., Shustrova O.V., Sivtsev V.S. The studying of the properties of the film compositions with gelatin and colchicine. Clinical Physiology of Blood Circulation. 2014; 3: 57–66 (in Russ).
- Bockeria L.A., Bockeria O.L., Sivtsev V.S., Novikova S.P., Salokhedinova R.R., Nikolashina L.N. et al. Experimental evaluation of the biodegradable film compositions based on gelatin with colchicine. Byulleten’ Eksperimental’noy Biologii i Meditsiny (The Bulletin of Experimental Biology and Medicine). 2016; 161 (3): 394–8 (in Russ).
- Bockeria L.A., Novikova S.P. Bioresorbable hydrogel polymer composition with biologically active substances (versions). Patent RU № 2519103, 2014 (in Russ).
- Yakubova O.S., Bekisheva A.A., Guseva D.A. Safety of fish gelatin. Food and marine biotechnology. In: V International Baltic Sea Forum. 2017; Kaliningrad: 1529–34 (in Russ).
- Yakubova O.S., Bekisheva A.A., Gusev D.A. Comparative characteristic of the properties of gelatin of different origin. In: IV International Baltic Sea Forum. 2017; Kaliningrad: 1528–38 (in Russ). 15. Yakubova O.S., Bekisheva A.A., Scientific substantiation physical properties of fish gelatin. Vestnik of Astrakhan State Technical University. Series: Fish Industry. 2018; 3: 132–40 (in Russ.). DOI: 10.24143/2073-5529-2017-3- 132-40
About the authors
- Leo A. Bockeria, Academician of the RAS and RAMS, President of Center, ORCID
- Svetlana P. Novikova, Dr. Biol. Sc., Professor, Head of the Laboratory, ORCID
- Regina R. Salokhedinova, Cand. Econ. Sc., Researcher, ORCID
- Natal’ya V. Nizova, Leading Technologist
- Aleksandra A. Orlova, Leading Technologist
- Natal’ya N. Samsonova, Dr. Med. Sc., Professor, Head of Laboratory, ORCID
- Aleksandr Yu. Gorodkov, Dr. Biol. Sc., Head of the Laboratory, ORCID
- Yuriy M. Tsygankov, Cand. Med. Sc., Researcher, ORCID
- Roman A. Serov, Dr. Med. Sc., Head of the Department, ORCID
- David R. Tevosov, Junior Researcher, ORCID
- Georgiy A. Khugaev, Junior Researcher, ORCID
- Olesya S. Yakubova, Cand. of Engineering, Head of the Laboratory
- Adelya A. Bekesheva, Cand. of Engineering, Senior Lecturer
